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Contrasting these studies, nhr-80 mutants exhibit an increased stearic acid to oleic acid ratio, yet a normal lifespan (Brock et al., fat-5 , -6 , or -7 single mutants do not show a shortened life span, albeit these mutants upregulate other desaturases, perhaps in a compensatory fashion (Brock et al., fat-4 mutants exhibit an increased lifespan (Lucanic et al., 2011), possibly through their effects on N -acylethanolamine (NAE) levels and endocannabinnoid signaling (see below)

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Mitochondrial dynamics imaging For mitochondrial visualization, human SH-SY5Y cells were transfected with the GFP-Mito plasmid using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturers protocol

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